Dr. P .R. Manish Kumar
Professor
Formerly, Co-ordinator & Head of the Department from 2008-10 ; 2012-14 & 2016 - 18.
Dept. of Biotechnology
University of Calicut
Awarded JRF by C.S.I.R. (Govt. of India) ; Awarded Ph.D. in 1991 [ University of Calicut ]; Awarded(Govt. of India) -Biotechnology Research Associateship (1992-’94) at Indian Institute of Science, Bangalore
Lecturer in College then affiliated to Univ. of Calicut – 1994-’96 , joined Dept. of Biotechnology University of Calicut as teaching faculty in 1996;Teaching courses in Cell & Developmental Biology Biology, Molecular Biology and Recombinant DNA Technology – M.Sc. Biotechnology Program ( Govt. of India – DBT sponsored National Program); M.Phil. and Ph.D. Program, University of Calicut; Guiding students under M.Phil and Ph.D.
Undergone Specialized Training in Electron Microscopy ( AIIMS, N. Delhi);Radiotracer techniques (BARC, Mumbai);Basic molecular biology and recombinant DNA techniques ( Univ. of Calicut; IISc Bangalore);Animal cell culture (Reliance Life Sciences, Navi Mumbai);Advances in Biotechnology ( TERI, N.Delhi)
Awarded ‘Best Subject Expert for the year 2000’ by University Grants Commission for the Educational video on DNA Fingerprinting entitled ‘DNA evidence’ produced by Audiovisual Research Centre ( AVRC)
Co- Principal Investigator in DBT ( Govt. of India) sponsored research project (2012-2015) with a total budget of Rs. 47.39 lakhs on ‘Screening for antiproliferative and anticancer drugs from endemic species of Zingiberaceae on human cancer cell lines’.
Membership- academic bodies: Served as Chairman, Board of studies ( Single Board) in Genetics, Member – Board of Studies in Biotechnology, Univ. of Calicut; Served as DBT (Govt. of India) nominee – Institutional Biosafety Committee - External subject Expert nominee– Research Admission Committee – IISR, Calicut;; Department of Biotechnology, VIT, Vellore;Member – Board of Studies ( Marine Biotechnology ) KUFOS –Kerala University of Fisheries and Ocean Studies, Panangad, Kochi.
Coordinator of DBT M.Sc. program and Bioinformatics facility & Head of the Dept - 2008 - 2010 & 2012 – 2014. Coordinator : UGC- ASC / HRDC Refresher courses – Biotechnology (2 courses) and Life Sciences ( 1 course )
Research Interests : Molecular Biology of Cell cycle regulation; DNA cassettes involved in bacterial drug resistance; novel anticancer drugs from plant; human cancer cell lines; DNA damaging agents ;biogenic nanoparticle production and their cellular effects
M.Phil / Ph.D guidance – several awarded as well as ongoing
Publications : ~50 nos ( National and International Journals)
Publications
60.T. Soumya, T. Lakshmipriya, Karel. D. Klika, P. R. Jayasree and P. R. Manish Kumar. Anticancer potential of rhizome extract and a labdane diterpenoid from Curcuma mutabilis plant endemic to Western Ghats of India. Scientific Reports. Nature Publishing Group. Impact factor: 3.998 (Accepted).
59.Ahlam, A.A., Shaniba, V.S., Jayasree, P.R. and Kumar, P.M., 2020. Spondias pinnata (Lf) Kurz Leaf Extract Derived Zinc Oxide Nanoparticles Induce Dual Modes of Apoptotic-Necrotic Death in HCT 116 and K562 Cells. Biological Trace Element Research, pp.1-24.
58.T. Lakmipriya, T. Soumya, P. R. Jayasree and P. R. Manish Kumar. (2019) Cytotoxic and apoptogenic effects of a bioactive fraction isolated from the leaves of a traditional medicinal plant Tridax procumbens. International journal of pharmaceutical sciences and research. November 2019; Vol. 10(11): 5075-5086. E-ISSN: 0975-8232; P-ISSN: 2320-5148. doi:10.13040/IJPSR.0975-8232.10(11).5075-86. Impact factor:0.83
57.Manju Suresh, Nithya N., Vimal K. P. , Jayasree P. R., Manish Kumar P. R. Mutational and phylogenetic analysis of nfxB gene in multidrug-resistant clinical isolates of Pseudomonas aeruginosa hyperexpressing MexCD-OprJ efflux pump. Advances in Microbiology .Scientific Research- an academic publisher. Vol. 9 No.12, December 2019 doi: 10.4236/aim.2019.912064 Impact Factor: 1.22
56.Manju Suresh, Sinosh Skariyachan, Nithya N., Jayasree P. R., Manish Kumar P. R. Mutational variation analysis of oprD porin gene in multidrug resistant clinical isolates of Pseudomonas aeruginosa. Microbial Drug Resistance. Mary Ann Liebert, Inc., publishers. Impact Factor: 2.397 (Accepted)
55 Ahlam Abdul Aziz, V. S. Shaniba, P. R. Jayasree . P. R. Manish Kumar (2019) Physico-chemical, photocatalytic and cytotoxicity evaluation of Annona muricata L. fruit extract derived zinc oxide nanoparticles in comparison to the commercial chemical version. Current Science, 117(9). 1492 – 1504. Impact factor-0.756
54. Book Chapter (accepted) entitled ‘Selective Targeting of Human Colon Cancer HCT 116 Cells by PhytoMediated Silver Nanoparticles’ by V. S. Shaniba, Ahlam Abdul Aziz, Jobish Joseph, P. R. Jayasree, and P. R. Manish Kumar – under Pharmaceutical Science and Technology series – Natural Products Chemistry (Academic Apple Press Inc. USA) – in production – Publn. - May 2020
53. Ahlam Abdul Aziz, Shaniba V.S, Jayasree, P.R. and Manish Kumar P.R.(2019) ' Physico-chemical, photocatalytic and cytotoxicity evaluation ov Annona muricata L.fruit extract derived zinc oxide nanoparticles in comparison to commercial chemical version'. (accepted)
52. Shaniba V.S, Ahlam Abdul Aziz, Jayasree, P.R. and Manish Kumar P.R.(2019) ‘Manilkara zapota(L.) P. Royen leaf extract derived silver nanoparticles induce apoptosis in human colorectal carcinoma cells without affecting human lymphocytes or erythrocytes’. Biological Trace Element Research (Springer Nature) – Published online on Mar 8. 2019; doi: 10.1007/s12011-019-1653-6. [Thomsun & Reuters IF – 2.361]
51. T. Soumya, P. R. Jayasree, M. Deepak & P. R. Manish Kumar (2019) ‘Chemical composition, antioxidant and antiproliferative activities of essential oil from rhizome and leaves of Curcuma mutabilis Škorničk., M. Sabu & Prasanthk., endemic to Western Ghats of India.’ Natural Product Research (Taylor and Francis Group) - https://doi.org/10.1080/14786419.2018.1533826. [Thomsun & Reuters IF – 1.928]
50. Manju Suresh, Nithya N, Jayasree PR, Vimal KP, Manish Kumar PR. (2018). Mutational analyses of regulatory genes, mexR, nalC, nalD and mexZ of mexAB-oprM and mexXY operons, in efflux pump hyperexpressing multidrug-resistant clinical isolates of Pseudomonas aeruginosa. World Journal of Microbiology and Biotechnology. Vol. 34: p. 83 (https://doi.org/10.1007/s11274-018-2465-0 Springer Nature Thomson & Reuters IF - 2.1)
49. Lakshmipriya, T., T. Soumya, P. R. Jayasree, P. R. Manish Kumar. Selective induction of DNA damage, G2 abrogation, and mitochondrial apoptosis by leaf extract of traditional medicinal plant Wrightia arborea in K562 cells. Protoplasma ( Springer Publication) – July 2017 (Doi:10.1007/s00709-017-1137-5) . Thomson & Reuters - Impact factor-2.87
48. Lakshmipriya, T., Soumya, T., Jayasree, P.R. & Kumar P.R.M. Antioxidant, antimicrobial and antiproliferative activities of leaf extracts of the Indian traditional medicinal plant Wrightia arborea. IJPSR. Vol 8,issue 3 March (2017) ISSN 0975-8232.
47. Shaniba, V. S., Aziz A.A. & Kumar, P.R.M. Phyto-mediated synthesis of silver nanoparticles from Annona muricata fruit extract, assessment of their biomedical and photocatalytic potential. IJPSR, Vol 8. Issue 1 (2017) ISSN 0975-8232.
46. Nithya,N, Rabindran, R.,Jayasree, P.R, Faisal, M. & Kumar, P.R.M. Analysis of beta-lactamase,blaNDM-1 phylogeny and plasmid replicons in multidrug-resistant Klebsiella. Indian J. Med. Res. (2017). Vol. 146. S38 – S45. ISSN 0971-5916. Wolters Kluwer Publication Thomson Reuters - Impact factor 2.06
45. Soumya, T., Lakshmipriya, T., Jayasree, P.R. & Kumar, P.R.M. (2016). Evaluation of total phenolics, antioxidant and antiproliferative activities of rhizome extracts from select Zingiberaceae species in South India. Int J. Phyt. Vol 8, issue 3 ISSN 0975-0185.
44. Manju, S., Nithya,N., Jayasree, P.R. & Kumar, P.R.M. (2016). Detection and prevalence of efflux pump-mediated drug-resistance in clinical isolates of multidrug-resistant Gram-negative bacteria from north Kerala, India. Asian J Pharm Clin Res. 9,324-327 ISSN 0974-2441
43. Nithya, N., Manju S., Jayasree P. R&., Kumar, P.R.M. (2016). Detection of blaNDM-1 and genetic relatedness in clinical isolates of Escherichia coli producing Extended spectrum β-lactamase from tertiary care centres in South India. Adv Microbiol. 6,125-132 ISSN 2165-3410
42. Nithya N, Lakshmipriya T, Jayasree PR, Kumar P. R. M. (2015).Drug resistance, Biofilm formation and occurrence of mrkABCDF operon in plasmid- bearing clinical isolates of E.coli and Klebsiella spp. from Kerala,India. Research journal of Pharmaceutical, Biological and Chemical Sciences 6:1044-1049. [ Impact factor: 0.33]
41. Iyyappan Rajan, Jayasree P. R and Kumar P. R. M. (2015). Zerumbone induces mitochondria-mediated apoptosis via increased calcium, generation of reactive oxygen species and upregulation of soluble histone H2AX in K562 chronic myelogenous leukemia cells. Tumor Biol (Published online May 31, (Springer: DOI: 10.1007/s13277-015-3583-z) [ Impact factor: 3.611]
40. Iyyappan Rajan, Remitha Rabindran, Nithya Narayanan, Lakshmipriya T, Jayasree P. R and Kumar P. R. M. (2014). Assesment of cell cycle phase specific effects of zerumbone on mitotically synchronous surface cultures of Physarum polycephalum. Protoplasma (Springer: DOI: 10.1007/s00709-013-0605-9) [ Impact factor: 3.171]
39. Iyyappan Rajan, Remitha Rabindran, Jayasree P. R and Kumar P. R. M. (2014). Antioxidant potential and oxidative DNA damage preventive activity of unexplored endemic species of Curcuma. Indian Journal of Experimental Biology 52:133-138 [Impact factor 1.3]
38. Iyyappan Rajan, Nithya Narayanan, Remitha Rabindran, P. R. Jayasree, P. R. Manish Kumar, (2013). Zingerone Protects Against Stannous Chloride-Induced and Hydrogen Peroxide-Induced Oxidative DNA Damage In Vitro. Biological Trace Element Research ( Springer:155:455–459, DOI 10.1007/S 12011-013-9801-X) [Impact factor 1.6]
37. Rajan Iyappan, Remitha Rabindran, P.R. Jayasree & Kumar P.R.M. (2013) Cell cycle inhibitory effects of leaf extract from Curcuma vamana M. Sabu & Mangaly on mitotically synchronous cultures of Physarum polycephalum Schw. Indian Journal of Experimental Biology 51: 81-87 [ Impact factor 1.3]
36. Remitha Rabindran, Rajan Iyappan, P.R. Jayasree & Kumar P.R.M. (2012). Involvement of 53 kDa protein in wortmannin-mediated abrogation of UV-induced G2 arrest in Physarum polycephalum. International Journal of Integrative Biology Vol.13, 2, p64-70 ISSN (online): 0973-8363,ISSN (print): 0974-2816.
35. Anas K, Jayasree P R, Vijayakumar T, Kumar P. R. M. (2008). In vitro antibacterial activity of Psidium guajava Linn. Leaf extract on clinical isolates of multidrug-resistant Staphylococcus aureus. Indian Journal of Experimental Biology 46: 41 [Impact factor 1.3]
34. Venu T, Vishwandham D, Jayasree P.R and Kumar P.R.M., (2003). Ethanolic extract of Bacopa Monniera (Brahmi) induces shortening of cell cycle durations in naturally synchronous Physarum Polycephalum , Curr. Sci. 85:3, 245. [Impact factor 0.9]
33. Jayasree P.R, Kumar P.R.M. and Nair V.R ,(2000). The plasmodia of Physarum polycephalum an elegant system to demonstrate the importance of genome integrity in traversing the G2/M checkpoint of the cell cycle , Curr. Sci. 78:9 101. [Impact factor 0.9]
32. Kumar P.R.M. and Nair V.R. (1993). Synergistic effect of Griseofulvin and ultraviolet irradiation on mitotic cycle durations in the plasmodia of Physarum polycephalum, Biomedical letters ( Faculty Press, Cambridge, England) 48, 137.
31. Kumar PRM and Nair V.R , (1992). A Comparative study of mitotic delay and inhibition of transcription in cells exposed to UV and heat shock: the possible use of mitotic delay duration as a parameter to assay the extent of damage and recovery , Curr. Sci. 62:3, 314. [Impact factor 0.9]
30. Kumar PRM and Nair V.R , (1991). Synergistic effect of heat-shock and UV irradiation on mitosis and protein synthesis in G2 phase plasmodia of Physarum polycephalum schew-reduction in UV induced mitotic delay in a pre shocked system , Indian J. Exp. Biol. 29, 305. [Impact factor 1.3]
29. Kumar PRM and Nair V.R , (1990). Effect of heat shock on the different phases of the
mitotic cycle in Physarum Polycephalum Curr. Sci. 59:12,615, [Impact factor 0.9]
Conference papers
28.Ahlam Abdul Aziz, Shaniba V.S., Manish Kumar P.R. Antimicrobial and antiproliferative potential of phyto-mediated zinc oxide nanostructures synthesized employing Annona muricata L. CRDB-2019 - International Conference on Research and Development in Biosciences held at Department of Biotechnology and Microbiology, Kannur University on 17-19 January 2019.
27.Soumya T., Lakshmipriya T., Jayasree P.R., Manish Kumar P.R. Phytochemical analysis, antioxidant and antiproliferative activities of organic solvents from Curcuma haritha – an endemic species from Western ghats of Kerala. CRDB-2019 - International Conference on Research and Development in Biosciences held at Department of Biotechnology and Microbiology, Kannur University on 17-19 January 2019 (Best Poster Award).
26.Ahlam Abdul Aziz, Shaniba V.S., Manish Kumar P.R. Genomic level interaction of biogenic zinc oxide nanostructures on prokaryotic and eukaryotic cell systems. Innopharm 3 - 3rd International conference on academic and industrial innovations: Transitions in Pharmaceutical, Medical and BIosciences held at Kala Academy, Goa on 22-23 October 2018 [Second prize]
25.Soumya, T., Jayasree, P.R., Manish Kumar P.R. Phytochemical analysis, antioxidant and antiproliferative activities of organic solvent extracts from Curcuma neilgherrensis leaves. International conference on Phytomedicine (ICPM 2018) held at Bharathiyar University, Coimbatore on 29-31 August 2018.
24. Soumya, T., Jayasree, P.R. & Kumar, P.R.M. ( 2018). Phytochemical analysis, antioxidant and antiproliferative activities of organic solvent extracts from curcuma neilgherrensis leaves. International conference on Phytomedicine. Bharathiyar University. Accepted.
23. Shaniba V.S, Ahlam Abdul Aziz, Dr. P. R Jayasree and Dr. P.R. Manish Kumar. (2018).Green synthesis of silver nanoparticles from Manikara zapota leaf extract and in-vitro evaluation of its biological activities. International conference on emerging trends in agriculture, food processing, engineering and Biotechnology held at Karunya institute of technology & science, Coimbatore, 21-23 February 2018.
22. Shaniba V.S, Ahlam Abdul Aziz, Dr. P. R Jayasree and Dr. P.R. Manish Kumar. (2018) Apoptogenic potential of biosynthesized silver nanoparticles on Human colon cancer HCT116 cell line. 30th Kerala Science Congress, Govt. Brennen College, Thalassery, 28-30 January, 2018.
21. Ahlam Abdul Aziz, V.S. Shaniba, Jayasree P. R. and Dr. P.R. Manish Kumar. (2017). Synthesis, Characterization and in-lab assessment of biofunctionalized zinc oxide nanostructures. International Symposium on nanomaterials for clean energy and health applications, 06-08 December 2017.
20. Shaniba V.S, Ahlam Abdul Aziz, Dr. P. R Jayasree and Dr. P.R. Manish Kumar. (2017). Annona muricata root extract-mediated biomimetic synthesis of silver nanoparticles: Characterization and biomedical applications. 27th Swadeshi Science Congress held at Amrita Viswa Vidyapeetham, Amrita University, Kollam. 07-09 November 2017
19. Manju Suresh , Jayasree P. R. & Manish Kumar P. R. (2017). Contribution of porins and beta-lactamases in carbapenem resistance among multidrug resistant Pseudomonas aeruginosa and their genetic relatedness. 27th Swadeshi Science Congress held at Amrita Viswa Vidyapeetham, Amrita University, Kollam. 07-09 November 2017.
18. Manju Suresh, Jayasree P. R, Manish Kumar P. R. (2017). Prevalence of MexAB-OprM and MexXY efflux systems in multidrug- resistant clinical isolates of Pseudomonas aeruginosa: A threat to environment. Indian Science Congress Association (ISCA) Coimbatore chapter held at Kongunadu arts and science college, Coimbatore. 09-11 October 2017 (Best paper award).
17. Ahlam Abdul Aziz, V.S. Shaniba and P.R. Manish Kumar. (2017). An invitro evaluation of phyto-derived zinc nanoparticles as a promising anti- cancer drug. Indian Science Congress Association (ISCA) Coimbatore chapter held at Kongunadu arts and science college, Coimbatore. 09-11. October 2017 (Best paper award).
16. Lakshmipriya T, Soumya T, Jayasree P.R. & Kumar P.R.M. (2017). Selective killing of human chronic myelogenous leukemic cells by a bioactive fraction isolated from leaves of the Ayurvedic medicinal plant Tridax procumbens. 29th Kerala Science congress, Marthoma College, Thiruvalla, Jan 28 – 30.
15. Soumya T, Tapas Pradhan, Lakshmipriya T, Jayasree P.R. & Kumar P.R.M. (2017). Enhancement of demethoxycurcumin mediated cell death by the checkpoint kinase inhibitor PD 407824 in K562 leukemic cell line – A preliminary report. 29th Kerala Science congress, Marthoma College, Thiruvalla, Jan 28 – 30.
14. Shaniba V.S, Ahlam Abdul Aziz & Kumar, P.R.M. (2017). Green synthesis of silver nanoparticles employing Manikara zapota leaf extract and evaluation of their antioxidant activities. International Conference on Advances in Functional Materials (ICAFM 2017). Anna University, Chennai, 6-8th Jan.
13. Ahlam Abdul Aziz , Shaniba V.S & Kumar, P.R.M. (2017). Pyto-mediated synthesis of photocatalytically active zinc oxide nanoparticles from fresh flowers of Cassia fistuala. International Conference on Advances in Functional Materials (ICAFM 2017). Anna University, Chennai, 6-8th Jan.
12. Soumya, T., Lakshmipriya, T., Jayasree, P.R.& Kumar, P.R.M. ( 2016). Antiproliferative activity of Curcuma mutabilis rhizome extracts against human cancer cell lines. National seminar on drug-discovery and cancer therapy – Dept. of Biochemistry and Molecular Biology, Pondicherry University 25-26 Feb.
11. Manju, Nithya, N., Jayasree, P.R. & Kumar, P.R.M. (2016). Prevalence and rapd analysis of various beta-lactamases- ESBL, ampC and MBL - among multi drug resistant pseudomonas aeruginosa clinical isolates from north Kerala. 28th Kerala Science Congress, University of Calicut, Jan 28-30.
10. Shaniba V.S, Ahlam Abdul Aziz. & Kumar, P.R.M. (2015) Green synthesis and Characterization of siver nano particles from Manikara zapota leaf broth. UGC sponsored National level conference on sustainable Biotechnology,E.M.E.A College of Arts and Science, Kondotti. Dec 16-17.
9. Ahlam Abdul Aziz, Shaniba V.S. & Kumar, P.R.M. (2015) Characterization and in Vitro analysis of Zinc Nanoparticles fabricated using Graviola root extracts. International conference on Nanostructured Polymeric Materials and Polymer Nanocomposites, M.G. University, Kottayam. Nov 13-15.
8. Shaniba, V.S., Aziz A.A. & Kumar, P.R.M. (2015) Biosynthesis of Silver nanoparticles using Annona Muricata fruit extract and assessment of its reducing power, International conference on Nanostructured Polymeric Materials and Polymer Nanocomposites, M.G. University, Kottayam. Nov 13-15.
7. Soumya T., Anju Lata Singh., P.R.Jayasree and P. R. Manish Kumar (2015).Antioxidant and Antiproliferative activities of different organic extracts of Curcuma rakthakanta rhizome and leaf.UGC sponsored National seminar on Recent developments in Cancer Biology, 22-24 July 2015,Organized by Department of Lifesciences,Pazhassiraja College,Pulpally,Wayanad.(First Prize in oral presentation).
6. Ahalam Abdul Aziz, Shaniba V. S., and Manish Kumar P. R. (2014). sol-gel synthesis and characterization of zinc nanoparticles from leaves of Annona muricata. Kerala Science Congress, Allappuzha, Jan 2015.
5. Shaniba V. S., Ahalam Abdul Aziz and Manish Kumar P. R. (2014). Green synthesis of silver nano particles from Annona muricata root extract and its antimicrobial activity. International Conference on Biosciences – State-of-the-art Advancements ( Proceedings of the Society for Education and Scientific Research ), Sept. 11 – 12. Kottayam. P. 76.
4. Lakshmipriya T., Nithya N., Jayasree P. R., and Manish Kumar P. R. (2014). In vitro screening of antimicrobial activity of Wrightia arborea against clinical isolates of multi- drug resistant bacteria. International Conference on Biosciences – State-of-the-art Advancements ( Proceedings of the Society for Education and Scientific Research ), Sept. 11 – 12. Kottayam.. P. 110.
3. Nithya N., Rajan I., Lakshmipriya T., Jayasree P. R., and Manish Kumar P. R. (2014). PCR- based screening to detect the prevalence of Class I integron in meropenem-resistant clinical bacterial isolates – presented in Kerala Science Congress Jan 28-31, Wayanad 2014 (Best Poster Award in Biotechnology)
2. Rajan Iyappan, Remitha Rabindran, P.R. Jayasree & Kumar P.R. M. Antioxidant, DNA protective activity and cellular effects of leaf extracts from Curcuma species (Zingiberaceae) endemic to South India, Paper presented in 6th International Symposium on the family Zingiberaceae, Sept 10 – 13, University of Calicut, Organized by Department of Botany, University of Calicut & Indian Association for Angiosperm Taxonomy(2012)
1. Rajan Iyappan, Remitha Rabindran, P.R. Jayasree & Kumar P.R.M. Paper presented in International Symposium on innovations in Free Radical Research and Experimental Therapeutics &5th Annual Convention of Association of Biotechnology and Pharmacy, Organized by Department of Biotechnology & School of Biotechnology and Health Sciences, Karunya University, Coimbatore December 7-9 (2011).
Accession numbers of DNA sequences deposited in GenBank (NCBI)
1. Manju,S., Nithya,N., Jayasree,P.R. and Manish Kumar,P.R. (2018) Pseudomonas aeruginosa strain Pa6 NalD (nalD) gene, complete cds. GenBank accession no. MG744560.
2. Manju,S., Nithya,N., Jayasree,P.R. and Manish Kumar,P.R. (2018) Pseudomonas aeruginosa strain Pa13 NalD (nalD) gene, complete cds. GenBank accession no. MG757449.
3. Manju,S., Nithya,N., Jayasree,P.R. and Manish Kumar,P.R. (2018) Pseudomonas aeruginosa strain Pa16 NalD (nalD) gene, complete cds. GenBank accession no. MG757450.
4. Manju,S., Nithya,N., Jayasree,P.R. and Manish Kumar,P.R. (2018) Pseudomonas aeruginosa strain Pa6 NalC (nalC) gene, complete cds. GenBank accession no. MG757451.
5. Manju,S., Nithya,N., Jayasree,P.R. and Manish Kumar,P.R. (2018) Pseudomonas aeruginosa strain Pa13 NalC (nalC) gene, complete cds. GenBank accession no. MG757452.
6. Manju,S., Nithya,N., Jayasree,P.R. and Manish Kumar,P.R. (2018) Pseudomonas aeruginosa strain Pa16 NalC (nalC) gene, partial cds. GenBank accession no. MG757453.
7. Manju,S., Nithya,N., Jayasree,P.R. and Manish Kumar,P.R. (2018) Pseudomonas aeruginosa strain Pa6 MexR (mexR) gene, partial cds. GenBank accession no. MG757454.
8. Manju,S., Nithya,N., Jayasree,P.R. and Manish Kumar,P.R. (2018) Pseudomonas aeruginosa strain Pa13 MexR (mexR) gene, partial cds. GenBank accession no. MG757455.
9. Manju,S., Nithya,N., Jayasree,P.R. and Manish Kumar,P.R. (2018) Pseudomonas aeruginosa strain Pa16 MexR (mexR) gene, complete cds. GenBank accession no. MG757456.
10. Manju,S., Nithya,N., Jayasree,P.R. and Manish Kumar,P.R. (2018) Pseudomonas aeruginosa strain Pa5 MexZ (mexZ) gene, complete cds. GenBank accession no. MG757457.
11. Manju,S., Nithya,N., Jayasree,P.R. and Manish Kumar,P.R. (2018) Pseudomonas aeruginosa strain Pa25 MexZ (mexZ) gene, complete cds. GenBank accession no. MG757458.
12. Manju,S., Nithya,N., Jayasree,P.R. and Manish Kumar,P.R. (2018) Pseudomonas aeruginosa strain Pa29 MexZ (mexZ) gene, partial cds. GenBank accession no. MG757459.
13. Manju,S., Nithya,N., Jayasree,P.R. and Manish Kumar,P.R. (2018) Pseudomonas aeruginosa strain Pa16 MexZ (mexZ) gene, partial cds. GenBank accession no. MG757460.
14. Manju,S., Nithya,N., Jayasree,P.R. and Manish Kumar,P.R. (2018) Pseudomonas aeruginosa strain Pa13 MexZ (mexZ) gene, partial cds. GenBank accession no. MG757461.
15- 42. The oprD gene sequences of MDR P. aeruginosa : MH122946-MH122954, MH135303-MH135311, MH142581-MH142588 (26 nos.)
43 – 45. The nfxB gene sequences of MDR P. aeruginosa : MH346508 - MH346510 (3 nos.)
46 - 71. The mexT gene sequences of MDR P. aeruginosa : MH397275-MH397298 (24 nos.)
72. Nithya,N., Remitha,R., Manju,S., Jayasree,P.R., Kumar,P.R.M. Escherichia coli strain EGE14 class 1 integron, partial sequence, Gen Bank: ACCESSION KU751767.
73. Nithya,N., Remitha,R., Manju,S., Jayasree,P.R., Kumar,P.R.M Escherichia coli strain EGE20 class 1 integron, partial sequence, Gen Bank :ACCESSION KU751768.
73. Nithya,N., Remitha,R., Manju,S., Jayasree,P.R., Kumar,P.R.M .Klebsiella sp. EGE5 class 1 integron, partial sequence, Gen Bank : ACCESSION KU751769.
74. Nithya,N., Remitha,R., Manju,S., Jayasree,P.R., Kumar,P.R.M Escherichia coli strain EGE25 class 1 integron, partial sequence,Gen Bank : ACCESSION KU751770.
75. Nithya,N., Remitha,R., Manju,S., Jayasree,P.R., Kumar,P.R.M. Escherichia coli strain EGE26 class 1 integron, partial sequence, Gen Bank : ACCESSION KU751771.
76. Nithya,N., Remitha,R., Jayasree,P.R., Kumar,P.R.M. Klebsiella sp. K27 New Delhi metallo-beta-lactamase (blaNDM) gene, partial cds, Gen Bank: ACCESSION KX090027.
77. Nithya,N., Remitha,R., Jayasree,P.R., Kumar,P.R.M. Klebsiella sp. K28 New Delhi metallo-beta-lactamase (blaNDM) gene, partial cds, Gen Bank : ACCESSION KX090028.
78. Nithya,N., Remitha,R., Jayasree,P.R., Kumar,P.R.M. Klebsiella sp. K1 New Delhi metallo-beta-lactamase (blaNDM) gene, partial cds, Gen Bank : ACCESSION KX090029.
79. Nithya,N., Remitha,R., Jayasree,P.R., Kumar,P.R.M. Klebsiella sp. K13 New Delhi metallo-beta-lactamase (blaNDM) gene, partial cds,Gen Bank : ACCESSION KX090030.
80. Nithya,N.,Manju, S., Jayasree,P.R., Kumar,P.R.M. Escherichia coli strain E7 New Delhi metallo-beta-lactamase (blaNDM) gene, blaNDM-1 allele, partial cds, Gen Bank: ACCESSION KX100581.
81. Nithya,N.,Manju, S., Jayasree,P.R., Kumar,P.R.M Escherichia coli strain E8 New Delhi metallo-beta-lactamase (blaNDM) gene, partial cds, Gen Bank : ACCESSION KX100582.
82. Nithya,N.,Manju, S., Jayasree,P.R., Kumar,P.R.M Escherichia coli strain E10 New Delhi metallo-beta-lactamase (blaNDM) gene, partial cds, Gen Bank : ACCESSION KX100583.
83. Nithya,N.,Manju, S., Jayasree,P.R., Kumar,P.R.M. Escherichia coli strain E23 New Delhi metallo-beta-lactamase (blaNDM) gene, partial cds, Gen Bank : ACCESSION KX100584.
84.Manishkumar.P.R, Manish.J.P.R & Rabindran.R.M. (2008). Physarum polycephalum sequence new. GenBank, EU 371923.
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